성인 골수 줄기세포의 조골세포로의 분화에서 유전자의 발현에 대한 연구
Exploring upregulated genes during osteogenic differentiation of hMSCs
안세경, 임재석, 권종진, 이의석, 장현석,
소속 상세정보
안세경 ( Ahn Se-Kyung ) - 고려대학교 의과대학 구강악안면외과학교실
임재석 ( Rim Jae-Suk ) - 고려대학교 의과대학 구강악안면외과학교실
권종진 ( Kwon Jong-Jin ) - 고려대학교 의과대학 구강악안면외과학교실
이의석 ( Lee Eui-Seok ) - 고려대학교 의과대학 구강악안면외과학교실
장현석 ( Jang Hyon-Seok ) - 고려대학교 의과대학 구강악안면외과학교실
KMID : 0355620080340010011
Abstract
Human bone marrow mesenchymal stem cells are thought to be multipotent cells, which are present in adult marrow, that can replicate as undifferentiated cells and that have the potential to differentiate to lineages of mesenchymal tissues, including bone, cartilage, fat, tenden, muscle, and marrow stroma. Cells that have the characteristics of human mesenchymal stem cells could be isolated from marrow aspirates of human and animals. This study was designed to identify and characterize genes specifically expressed by osteogenic supplements -treated cells by suppression subtractive hybridization(SSH) method. The results were as follows: 1. 2 genes were upregulated genes in osteogenic diffeentiation of hMSCs, which is further proved by Northern blot analysis. 2. IGFBP-2 has been identified playing an important role in bone formation. 3. HF1 was also upregulated during osteogenic differentiation, but its role in bone formation is not clear yet
키워드
Bone marrow mesenchymal stem cells;Osteogenic supplements;Suppression subtractive hybridization(SSH);IGFBP-2;HF1
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